|
KMID : 0385520110240050345
|
|
Analytical Science & Technology
2011 Volume.24 No. 5 p.345 ~ p.351
|
|
|
Determination of finasteride in human serum by LC-MS/MS
|
|
Nam Hye-Seon
Nam Kyong-Hee
Jung Su-Hee
Lee Jang-Woo
Kang Jin-Yeong
Hong Soon-Keun
Kim Tae-Sung
Kang Tae-Seok
Yoon Hae-Jung
Lee Kwang-Ho
Lee Gyu-Seek
|
|
|
Abstract
|
|
|
|
A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS) method was developed and validated for the determination of finasteride in human serum. Beclomethasone was used as internal standard (IS) and liquid-liquid extraction (LLE) using methyl tert-butyl ether (MTBE) was carried out to isolate analyte. The mass transitions monitored in multiple reaction monitoring (MRM) in positive ion mode were m/z 373.2→305.2 for finasteride and m/z 409.3→391.2 for IS. Retention times of finasteride and IS were 5.81 and 5.46 min, respectively. The limit of quantitation (LOQ) was 0.1 ng/mL and the calibration curve showed good linearity in the range of 0.1~20.0 ng/mL (R2=0.9997). The intra-day assay precision and accuracy were in the range 6.3~10.6% and 97.3~103.6%, respectively, and the inter-day assay precision and accuracy were in the range 0.8~5.2% and 99.8~102.5%, respectively. The sample extract recovery of the method was 80~83%.
|
|
|
KEYWORD
|
|
|
Finasteride, LC-MS/MS, human serum
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|